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FLAGIPT1
Sigma
FLAG® Immunoprecipitation Kit
Description
| Application | Sigma’s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with ANTI-FLAG®-M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays. |
| Features and Benefits | • Utilizes highly specific ANTI-FLAG M2 affinity gel • No preliminary steps or calibrations • 3X FLAG™ peptide provides efficient and gentle elution through direct competition |
| Legal Information | ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| 3xFLAG is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. | |
| FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| usage | kit sufficient for 50 reactions |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
| Hazard Codes | Xn |
| Risk Statements | 20/22-36/37/38-42 |
| Safety Statements | 23-26-36 |
